Kinetics of Bio-ethanol production on the molasses-based medium by Saccharomyces cerevisiae

نوع المستند : المقالة الأصلية

المؤلفون

Agric. Microbiology Dept., Fac. of Agric., Ain Shams Univ

المستخلص

Bioethanol           is a renewable and environmentally friendly biofuel because it is produced from renewable sources such as sugarcane molasses. One strategy for lowering production costs and making ethanol fuel economically competitive with fossil fuels could be to use overland yeast with somnolence and ethanol resistance. and low nutritional requirements This work focuses on the kinetics of ethanol production by Saccharomyces cerevisiae on untreated or treated molasses-based medium with the development of a mathematical model considering the effect of substrate concentration, inoculum size, and pretreatment of molasses on the growth rate, substrate consumption, and product concentration. Experiments were carried out in batch mode, with substrate concentration varying from 100 to 250 g L−1   and inoculum size from 1 to 4 gL-1.. It was discovered that there were significant effects on cell growth, substrate utilization, and ethanol production rates. The kinetic parameters were calculated using linear and non-linear regression methods. A Monod model was applied to obtain a more accurate fitting of kinetic parameters. The parameters such as maximum specific growth rate (µmax), saturation constant (Ks), substate to biomass (Yx/s), the  substrate to product (Yp/s), cell to product factor (YP/X)and the important parameter of fermentation efficiency (FE) (YX) was shown to be dependent on substrate concentration. The best concentration of molasses-based medium was given the highest bioethanol concentrations and fermentation efficiency was 150 and 250 gL-1 on untreated and treated molasses-based medium, respectively.

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