Influence of using of saponin-carbopol mixture adjuvant on the potency of freeze-dried Bovine Ephemeral Fever virus vaccine

نوع المستند : المقالة الأصلية

المؤلفون

Veterinary Serum and Vaccine Research Institute Abbasia, Cairo Department of Pet Animal Vaccine Research

المستخلص

تهدف هذه الدراسة إلى استبيان تأثير الکاربابول کمساعد للقاح حمى الثلاثة أيام الذى يتم تثبيطه وقتالتحصين باستخدام الصابونين  حيث تم  تحضير الکربابول بثلاث ترکيزات مختلفة (٫0٫1-0٫2-0٫5٪)  مع مادة الصابونين بنسبة ثابتة (0٫2ميکروجرام/مل) کمذيب خليط للقاح0 وقد تبين أن مادة الکاربابول آمنة عند حقنها فى العجول ضمن اختبار السلامة لهذه المادة کما تم تحصين  ثلاث مجموعات العجول البقرى باللقاح بعد اذابته بالمذيب المرکب من المادتين معا بالنسب المذکورة کل على حده  والمجموعة الرابعة باللقاح بعد اذابته بمادة الصابونين فقط بالاضافة الى مجموعة ترکت دون تحصين کضابط للتجربة0  هذا وقد تم تتبع المستويات المناعية فى الحيوانات المحصنة على مدارفترات متعاقبة باستخدام اختبار المصل المتعادل واختبار الاليزا حيث أظهرت نتائج هذه الاختبارات ان استخدام مذيب الکربابول بترکيز( 0٫2أو0٫5٪) مع مادة الصابونين (0٫2ميکروجرام/مل)للقاح حمى الثلاث ايام المجفد قد اعطت أعلى مستوىات مناعية (3٫12- 3٫14 لو2 تتابعا) , والکثافة الضوئية (3٫3995-  3٫5026 تتابعا )عنها فى حالة استخدام الصابونين وحده (2٫68 لو2)  والکثافة الضوئية (2٫9432) ولذا يوصى بأستخدام الصابونين مضافا إليه مادة الکربابول کمحفز مناعى للقاح حمى الثلاث ايام المجفد0       
 
ABSTRACT:
Identification of optimal complex adjuvant and bovine ephemeral fever (BEF) antigen combination to elicit potent, protective, and long lasting immunity has been a major challenge for improvement of the vaccines potency againstbovine ephemeral fever virus. In this study, three different concentrations (0.1, 0.2 and 0.5%) of a cross-linked polyanionic carbopol combined with a constant concentration (0.2 µg /ml) of saponin were used as solvents for the freeze dried BEF vaccine. The immunogenicity of these formulae was evaluated through application of serum neutralization test (SNT) and enzyme linked immunosorbent assay (ELISA) on serum samples obtained from different vaccinated groups of cattle in comparison with those obtained from cattle vaccinated with the BEF vaccine reconstituted in saponin diluent only. The results showed that the usage of (0.2% or 0.5%) of carbopol with (0.2 µg /ml) of saponin as a diluent for the freeze dried BEF vaccine induced the highest levels (3.12 and 3.14 Log2 respectively) of specific bovine ephemeral fever antibodies with long lasting immunity in vaccinated cattle. Thus, it could be recommended to use this diluent (carpobol plus saponin) as adjuvant to bovine ephemeral fever virus vaccine.

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