Influence of using of saponin-carbopol mixture adjuvant on the potency of freeze-dried Bovine Ephemeral Fever virus vaccine

نوع المستند : المقالة الأصلية

المؤلفون

Veterinary Serum and Vaccine Research Institute Abbasia, Cairo Department of Pet Animal Vaccine Research

المستخلص

تهدف هذه الدراسة إلى استبيان تأثير الکاربابول کمساعد للقاح حمى الثلاثة أيام الذى يتم تثبيطه وقتالتحصين باستخدام الصابونين  حيث تم  تحضير الکربابول بثلاث ترکيزات مختلفة (٫0٫1-0٫2-0٫5٪)  مع مادة الصابونين بنسبة ثابتة (0٫2ميکروجرام/مل) کمذيب خليط للقاح0 وقد تبين أن مادة الکاربابول آمنة عند حقنها فى العجول ضمن اختبار السلامة لهذه المادة کما تم تحصين  ثلاث مجموعات العجول البقرى باللقاح بعد اذابته بالمذيب المرکب من المادتين معا بالنسب المذکورة کل على حده  والمجموعة الرابعة باللقاح بعد اذابته بمادة الصابونين فقط بالاضافة الى مجموعة ترکت دون تحصين کضابط للتجربة0  هذا وقد تم تتبع المستويات المناعية فى الحيوانات المحصنة على مدارفترات متعاقبة باستخدام اختبار المصل المتعادل واختبار الاليزا حيث أظهرت نتائج هذه الاختبارات ان استخدام مذيب الکربابول بترکيز( 0٫2أو0٫5٪) مع مادة الصابونين (0٫2ميکروجرام/مل)للقاح حمى الثلاث ايام المجفد قد اعطت أعلى مستوىات مناعية (3٫12- 3٫14 لو2 تتابعا) , والکثافة الضوئية (3٫3995-  3٫5026 تتابعا )عنها فى حالة استخدام الصابونين وحده (2٫68 لو2)  والکثافة الضوئية (2٫9432) ولذا يوصى بأستخدام الصابونين مضافا إليه مادة الکربابول کمحفز مناعى للقاح حمى الثلاث ايام المجفد0       
 
ABSTRACT:
Identification of optimal complex adjuvant and bovine ephemeral fever (BEF) antigen combination to elicit potent, protective, and long lasting immunity has been a major challenge for improvement of the vaccines potency againstbovine ephemeral fever virus. In this study, three different concentrations (0.1, 0.2 and 0.5%) of a cross-linked polyanionic carbopol combined with a constant concentration (0.2 µg /ml) of saponin were used as solvents for the freeze dried BEF vaccine. The immunogenicity of these formulae was evaluated through application of serum neutralization test (SNT) and enzyme linked immunosorbent assay (ELISA) on serum samples obtained from different vaccinated groups of cattle in comparison with those obtained from cattle vaccinated with the BEF vaccine reconstituted in saponin diluent only. The results showed that the usage of (0.2% or 0.5%) of carbopol with (0.2 µg /ml) of saponin as a diluent for the freeze dried BEF vaccine induced the highest levels (3.12 and 3.14 Log2 respectively) of specific bovine ephemeral fever antibodies with long lasting immunity in vaccinated cattle. Thus, it could be recommended to use this diluent (carpobol plus saponin) as adjuvant to bovine ephemeral fever virus vaccine.

المراجع

1. Murphy, F.A.; Gibbs, E. P.J.; Horzinek, M. C. and Studdert, M.J. (1999): Veterinary Virology, Third Edition. Copyright by Academic Press.
2. Burgess GW and Spradbrow PB (1977): Studies on the pathogenesis of bovine ephemeral fever. Aust Vet J., 53: 363-368. Doi: 10.1111/j. 1751 0813.1977.tb07952.x. PubMed: 337960.
3. Walker PJ (2005): Bovine ephemeral fever in Australia and the world. Curr Top Microbiol Immunol, 292: 57-80. PubMed: 15981468.
4. Sewell, M.M.A. and Brocklesby,B.R (1990): Handbock of Animal Disease in the Tropics, 4th Edition, Bailliere Tindall, London.
5. Fearon, D.T., (1997): Seeking wisdom in innate immunity. Nature, 388 (6640):323-324.
6. Bomford, R.., (1998): Will adjuvants be needed for vaccines of the future? In: Brown, F., Haaheim, L.R. (Eds.), Modulation of the Immune Response to Vaccine Antigens, Vol. 92. Development in Biological Standardization, Basel, Karger, p.13-17.
7. Carbomer 934P. USP XXII, NF XVII. United States Pharmacopeial Convention Inc., Rockville, Maryland, 1990: 1911.
8- Oda, K., Matsuda, H., Murakami, T., Katayama, S., Ohgitani, T and Yoshikawa, M., (2000): Adjuvant and haemolytic activities of 47 saponins derived from medicinal and food plants. Biol. Chem., 381(1):67-74.
9. Jie, Y.H., Cammisuli, S.,and Baggliolini, M., (1984): Immunomodulatory effects of Panax ginseng CA Meyer in the mouse. Agents Actions, 15 (3-4):386-391.
10. Kensil, C.R., (1996). Saponins as vaccine adjuvants. Crit. Rev. Ther. Drug Carrier Syst., 13(1-2):1-55.
11. Soad, M. Soliman; Taha, M.M.; Samir, S.S. and Daoud, A. M. (2001): Isolation and identification of BEF virus in Egypt, Beni-Suef Vet. J. Vol. XI. No. (2), 24-32.
12. Azab, A. M, Khodeir, M.H.; Attyat, M. Kotb and El- Gallad, S. B. Kh. (2002): Susceptibility of different cell culture to bovine ephemeral virus. 6thVet. Med. Conf. Hurghada; 41-55.
13. Al-Behwar, A.M.; Nermein, G. Shafeik; Saad, M.A.; Ibrahim, M.M.; Magda, S. Mohamed; Anhar Abd El-Moety and Khodeir, M.H. (2010): Comparative evaluation of the potency of traditionally inactivated bovine ephemeral fever vaccine and that one inactivated on the time of vaccination. 14th Sci.Cong. Fac. Vet. Med. Assiut Univ., Egypt; 51-61.
14. Macpherson, I.A. and Stocker, M.G.P. (1962): Polyoma transformation of hamster cell clones: an investigation of genetic factors affecting cell competence Virology, 16:147.
15. Amoros, M.; Fauconnier, B. and Girre, R.L. (1987): In vitro antiviral activity of a saponin from Anagallis   arvensis, Primulaceae, against herpes simplex virus and poliovirus. Antiviral  Research, Vol.8, Issue 1, 13-25.
16- Code of Federal Regulation of USA, (1986):  Published by the Office of the Federal Register National Archives and Record Administration.  Animal and Animal products.
17. Rossiter, P.B.; Jessett, D.M. and Taylor, W.P.(1985): Microneutralization system for use with different strains of peste des petits ruminant's virus.  Top.  Anim. Hith. Prod., 17(2): 75-81.
18-Wagner, G.G., Card, J.L.,and Cowan, K.M., (1969): Plum Island Animal Disease Laboratory, Animal Disease and Parasite Research Division Agricultural Research Service. Department of Agricultural, Greanport, New York, USA.
19- Zakrzewski, H., Cybinski, D.H.,and Walker, P.J.(1992): A blocking ELISA for the detection of specific antibodies to bovine ephemeral fever virus. J. Immunol. Methods, 151, 289-297.
20- de Oliveira, C.A.C., Perez, A.C., Merino, G., Prieto, J.G.,and Alvarez, A.I., (2001): Protective effects of Panax ginseng on muscle injury and inflammation after eccentric exercise.Comp. Biochem. Physiol., 130(3):369-377.
21- Haridas, V., Arntzen, C.J.,and Gutterman, J.U., (2001): Avicins, a family of triterpenoid saponins from Acacia victoria (Bentham), inhibit activation of nuclear factor-kappa B by inhibiting both its nuclear localization and ability tobind DNA. Proc. Natl. Acad. Sci. USA, 98(20):11557-11562.
22- Delmas, F., Di Giorgio, C., Elias, R., Gasquet, M., Azas, N.,Mshvildadze, V., Dekanosidze, G., Kemertelidze, E.,and Timon-David, P., (2000): Antileishmanial activity of three saponins isolated from ivy, alpha-hederin, beta-hederin and hederacolchiside A(1), as compared with their action on mammalian cells cultured in vitro. Planta Medica, 66(4):343-347.
23-Yui, S., Ubukata, K., Hodono, K., Kitahara, M., Mimaki, Y., Kuroda, M., Sashida, Y., and Yamazaki, M., (2001): Macrophage-oriented cytotoxic activity of novel triterpene saponins extracted from roots of Securidaca inappendiculata. Int. Immunopharmacol., 1(11):1989-2000.
24-Duk Kyung Kim,A Hyun S. Lillehoj,AD Sung Hyen Lee,A Paul Dominowski,B Robert J. Yancey,B and Erik P. Lilleho (2012): Effects of Novel Vaccine/Adjuvant Complexes on the Protective Immunity AgainstEimeria acervulina and Transcriptome Profiles. Avian Diseases, 56:97–109, 2012
25- Krashias G, Simon AK, Wegmann F, Kok WL, Ho LP ,and Stevens D, (2010): Potent adaptive immune responses induced against HIV-1 gp140 and influenza virus HA by a polyanionic carbomer. Vaccine, 28(13):2482–2489.
26- Gartlana, , George Krashiasa, Frank Wegmanna,1, William R. Hillsona,Erin M. Schererb, Philip D. Greenbergb, Stephanie C. Eisenbarthc, Amin E. Moghaddama, and Quentin J. Sattentauaa Sir (2016):  Sterile inflammation induced by Carbopol elicits robust adaptive immune responses in the absence of pathogen-associated molecular patterns Kate H Vaccine 34 2188–2196
27-Diamantstein T, Wagner B, Beyse I, Odenwald MV, and Schulz G. (1971): Stimulation of humoral antibody formation by polyanions: I. The effect of polyacrylic acid on the primary immune response in mice immunized with sheep red blood cells. Eur J Immunol, 1:335–40.
28- Gall D, Knight PA, and  Hampson F. (1972): Adjuvant activity of polyelectrolytes. Immunology; 23:569–75.
29- Gualandi GL, Losio NM, Muratori G, and Foni E. (1988): The ability by different preparations of porcine parvovirus to enhance humoral immunity in swine and guinea pigs. Microbiologica, 11:363–9.
30- Tollersrud T, Norstebo PE, Engvik JP, Andersen SR, Reitan LJ, and  Lund A (2002): Antibody responses in sheep vaccinated against Staphylococcus aureus mastitis: a comparison of two experimental vaccines containing different adjuvants. Vet Res Commun, 26:587–600.
31- Lai RP, Seaman MS, Tonks P, Wegmann F, Seilly DJ, and Frost SD, (2012): Mixed adjuvant formulations reveal a new combination that elicit antibody response comparable to Freund’s adjuvants. PLoS  ONE 7.
32- Dey AK, Burke B, Sun Y, Hartog K, Heeney JL, and Montefiori D, (2012): Use of a polyanionic carbomer, Carbopol971P, in combination with MF59, improves antibody responses to HIV-1 envelope glycoprotein. Vaccine, 30:2749–59.
 
 
 
 
 
 
 
 
 
 
                         
                                                                                                                            
                       
 
 

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