Effect of Listeria monocytogenes on Food and Water Sources in Buffaloes' Farms

Mansour, Saad Samir Nasr; Abd El Khalek, Hanaa Mohammed M.; Nagib, Hanan Ezzat; Elias, Randa Sami Farag; Sabra, Sherifa Mostafa M.; Moshref, Badia Saad El Sayed; Zarea, Zizet Z.

doi:10.21608/asajs.2022.246482

Effect of Listeria monocytogenes on Food and Water Sources in Buffaloes' Farms

نوع المستند : المقالة الأصلية

المؤلفون

Bacteriology Dept., Animal Health Research Institute (AHRI), Agricultural Research Center (ARC)

10.21608/asajs.2022.246482

المستخلص

Listeria monocytogenes from Listeria genus, which as saprophytes, but causes systemic infections as pathogenic bacteria. Listeria monocytogenes is widely spread in environment, caused infection of farm animals during fields grazing leading to contamination by wildlife, or manure. There was a close relation between improperly farm environment originated from contaminated crops and listeriosis. It causes animals infection with is not associated with clinical symptoms then the animal is flaking Listeria monocytogenes in feces. Listeria species' identification is a serious to gain the ecological Listeria virulence influence. The samples understudy consisted of animal stool, mattress, ration and water (n = 30) were for each. All samples were exposed to bacterial check. The results obtained that Listeria species was isolated with a general occurrence of 33.3%. The stools had 17.5%, mattress 7.5%, ration 5.0%, and water 3.3%. Meanwhile the occurrence of Listeria monocytogenes was overall 20.0% included (10.0%, 5.0%, 2.5%, and 2.5 %) as the same aforementioned samples. The PCR-technique was applied for detection of Listeria monocytogenes virulence genes using primers groups. Those genes were hlyA (hemolysin A gene), inlA (internalin A), prfA. The results obtained the present of all virulence genes. Listeria monocytogenes susceptibility to antimicrobial agents usually used for farms treatment was determined for animal stools Listeria monocytogenes isolates. The results showed that the generally sensitive rate was 70.0%. The100.0% was sensitive to Ampicillin, Amoxicillin, Erythromycin, and Tetracycline followed by Ofloxacin 75.0%. But the 50.0% was sensitive to Penicillin G, Cephalexin, and Gentamycin, finally Lincomycin was resistant. It was concluded that the Listeria monocytogenes were isolated from understudy samples as noticeable degrees. Also, virulence genes were presented in the isolated Listeria monocytogenes. That powerful proved the attendance of Listeria monocytogenes trendy a hidden contamination in animal farm. So, that could affect the animal health and animal productions. It was recommended that the undertake to follow the "Farm Health Conditions" and follow-up to decrease the microbial contamination. As well fix the animal vaccinations to maintain the animal health and livestock.

الكلمات الرئيسية

المراجع

Ahmed, S., Kamar, M., Torky, A. (2020). Listeria species isolated from animal origin food and its susceptibility to antibiotic and biofilm formation. Alexandria Journal for Veterinary Sciences, 67(1).‏
Al-Shabi, M., Abuhamdah, A. (2022). Using deep learning to detecting abnormal behavior in internet of things. Int. J. Electrical & Computer Engineering (2088-8708), 12(2).‏ ‏
Aziz, A., Lafta, J. (2022). Developing multiplex PCR for the rapid and simultaneous detection of Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae associated with sheep respiratory tract infections. , Pp, 1-7.‏
Bording-Jorgensen, M., Parsons, B., Szelewicki, J., Lloyd, C., Chui, L. (2022). Molecular detection of non-O157 Shiga Toxin-Producing Escherichia coli (STEC) directly from stool using Multiplex qPCR Assays. Microorganisms, 10(2), 329.‏
Cao, X., Wang, Y., Wang, Y., Ye, C. (2018). Isolation and characterization of Listeria monocytogenesfrom the black-headed gull faeces in Kunming, China. Infect. Public Health, 11(1), 59-63.
Chiara, M., Caruso, M., D’Erchia, M., (2015). Comparative genomics of Listeriasensu lato: Genus-wide differences in evolutionary dynamics and the progressive gain of complex, potentially pathogenicity-related traits through lateral gene transfer. Genome Biol Evol., 7(8), 2154-2172.
de Faria, S., Gonçalves, U., Soares, C., Barbosa, H., Saliba, W., de Souza, A., de Avelar, M. (2022). Impact assessment of different DNA extraction methods for non-invasive molecular diagnosis of tegumentary leishmaniasis. Acta Tropica, 227, 106275.‏
Deneer, G., Boychuk, I. (1991). Species-specific detection of Listeria monocytogenes by DNA amplification. App.& Environ. Micro., 1991, 606-609.
El-Baz, H., Elsayed, M. (2021). Prevalence of Listeria monocytogenes in farm milk and some dairy products with comparing its sensitivity to different antibiotics and H₂O₂ loaded on Silver aanoparticles in Dakahlia Governorate, Egypt. Alex. J. Vet. Sci., 68(1).‏
El-Gohary, H., Mohamed, A., Ramadan, H., Abuhatab, A. (2018). Zoonotic and molecular aspects of Listeria species isolated from some farm animals at Dakahlia Province in Egypt. Alex. J. Vet. Sci., 58(1), 208-214.
EL-Naenaeey, S., Abdelwahab, A., Merwad, A., Abdou, H. (2019). Prevalence of Listeria species in dairy cows and pregnant women with reference to virulotyping of Listeria monocytogenes in Egypt. Zagazig Vet. J., 47(3), 248-258.‏
El-Sayed, M., Moustafa, E., Abdeen, E., Amin, S. (2019). Simultaneous detection of Brucella, Leptospira, Mycoplasma and Listeria species by Multiplex-PCR. Alex. J. Vet. Sci., 61(2).‏
Esposito, C., Cardillo, L., Borriello, G., Ascione, G., Valvini, O., Galiero, G., Fusco, G. (2021). First detection of Listeria monocytogenes in a buffalo aborted foetus in Campania Region (Southern Italy). Frontiers in Vet. Sci., Pp, 657.‏
Fallah, A., Saei-Dehkordi, S., Rahnama, M., Tahmasby, H., Mahzounieh, M. (2012). Prevalence and antimicrobial resistance patterns of Listeriaspecies isolated from poultry products marketed in Iran. Food Control, 28(2), 327-332.
Farag, H., Abdallah, M., Nossair, M. (2021). Prevalence of Listeriosis in some farm animals. Damanhour J. Vet. Sci., 6(1), 17-20.‏
Garvey, M. (2019). Food pollution: A comprehensive review of chemical and biological sources of food contamination and impact on human health. Nutrire, 44(1), 1-13.‏
Ghetas, M., Sedeek, M., Fedawy, S., Bosila, A., Mekky, M., Elbayoumi, M., Amer, M. (2022). Detection of intestinal fungi in chickens naturally infected with infectious bursal disease. Adv. Anim. Vet. Sci., 10(3), 514-520.‏
Govindkumar, B., Kavyashree, B., Patel, K., Sasidharan, K., Arumugam, S., Thomas, L., Acharya, K. (2022). Ex-Ex Primer: An experimentally validated tool for designing oligonucleotides spanning spliced nucleic acid regions from multiple species. J. Biotechn., 343, 1-6.‏
Ha, M. (2021). Management of infectious animal diseases:Tthe Korean experience. Int. J. Emergency Management, 17(1), 1-16.‏
Hoorfar, J. (2011). Rapid detection, characterization, and enumeration of foodborne pathogens. Apmis., 119, 1-24.‏
Humski, A., Mitak, M., Mikulić, M., Stojević, D., Šimpraga, B., Krstulović, F., Jurinović, L. (2022). Prevalence of Listeria monocytogenes and other Listeria species in gulls feeding at a landfill in Zagreb, Croatia. Vet. Stanica, 53(4), 361-367.‏
Law, F., Ab Mutalib, S., Chan, G., Lee, H. (2015). An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food. Frontiers in Micro., 6, 1227.‏
Liu, D., Lawrence, L., Austin, W., Ainsworth, J. (2007). A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes. J. Micro. Methods 71 (2007), 133-140.
Maffert, P., Reverchon, S., Nasser, W., Rozand, C., Abaibou, H. (2017). New nucleic acid testing devices to diagnose infectious diseases in resource-limited settings. European J. Clin. Micro. & Inf. Dis., 36(10), 1717-1731.‏
Manyi-Loh, C., Mamphweli, S., Meyer, E., Okoh, A. (2018). Antibiotic use in agriculture and its consequential resistance in environmental sources: Potential public health implications. Molecules, 23(4), 795.‏
Mazza, R., Piras, F., Ladu, D., Putzolu, M., Consolati, G., Mazzette, R. (2015). Identification of Listeriaspecies strains isolated from meat products and meat production plants by multiplex polymerase chain reaction. J. Food Saf., 4(4), 5498.
Nasrin, S., Hegerle, N., Sen, S., Nkeze, J., Sen, S., Permala-Booth, J., Tennant, M. (2022). Distribution of serotypes and antibiotic resistance of invasive Pseudomonas aeruginosa in a multi-country collection. BMC Micro., 22(1), 1-12.‏
Odu, N., Okonko, O. (2017). Prevalence and antibiotic susceptibility of Listeria monocytogenesin retailed meats in Port Harcourt Metropolis, Nigeria. Public Health Res., 7(4), 91-99.
Osman, M., Kappell, D., Fox, M., Orabi, A., Samir, A. (2020). Prevalence, pathogenicity, virulence, antibiotic resistance, and phylogenetic analysis of biofilm-producing Listeria monocytogenes isolated from different ecological Niches in Egypt: Food, Humans, Animals, and Environment. Pathogens, 9(1), 5.‏
Rodríguez-Melcón, C., Alonso-Calleja, C., García-Fernández, C., Carballo, J., Capita, R. (2022). Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for twelve antimicrobials (biocides and antibiotics) in eight strains of Listeria monocytogenes. Biology, 11(1), 46.‏
Saleh, E., Elboudy, A., Elsayed, A., Ali, E. (2021). Molecular characterization of listeria monocytogenes isolated from raw milk and some dairy products at local markets in Damanhour City, Egypt. Damanhour J. Vet. Sci., 6(1), 1-6.‏
Trinh, L., Lee, Y. (2018). Glass-polytetrafluoroethylene-glass based sandwich microdevice for continuous-flow polymerase chain reaction and its application for fast identification of foodborne pathogens. Talanta, 176, 544-550.‏
Weller, D., Wiedmann, M., Strawn, K., Schaffner, W. (2015). Spatial and temporal factors associated with an increased prevalence of Listeria monocytogenesin spinach fields in New York state. Environ. Micro., 81(17), 6059-6069.
Zaki, M., Babashamsi, P., Shahrir, H., Milad, A., Abdullah, H., Hassan, A., Yusoff, M. (2021). The impact of economic analysis methods on project decision-making in Airport Payment management. J. Techno., 83(3), 11-19.‏