Effect of Drought and Salicylic Acid on Vegitive Growth and Prolin Accumulation of Two Cowpea (Vigna unguiculata L.) Plants

Awni, Dilzheen Sami; Ibrahim, Diaa Ayoub

doi:10.21608/asajs.2022.228842

Effect of Drought and Salicylic Acid on Vegitive Growth and Prolin Accumulation of Two Cowpea (Vigna unguiculata L.) Plants

نوع المستند : المقالة الأصلية

المؤلفون

¹ Assistant researcher, Ecology Department, Faculty of Science, Univ. of Zakho.

² Prof. Dr. Scientific Research Center, College of Science Univ. of Duhok

10.21608/asajs.2022.228842

المستخلص

Current study was conducted during one growing season from March to July, 2021 in local farm under field conditions via plastic pots, in Biology Department/ Faculty of Science / University of Zakho -Kurdistan Region of Iraq. The experiment comprises studying soaking two local cowpea seeds, Iraqi and American cultivar in both 250 ppm of salicylic acid and distal water for four hours separately before planting. In addition to utilizing foliar spraying of 250 ppm of salicylic acid at three stages, vegetative (4^th true leaf stage), starting flowering stage and without spraying as a control treatment. and the effects of drought in three levels, control with continuous irrigation, three and five days of water deficit at flowering stage, followed by continuous daily irrigation (75% from field capacity) till the harvest date of the experiment. The results showed that immersion the seeds will promote seed germination percentage, plant length, number of flowers, shoot fresh weight, roots fresh weight, roots length, shoots dry weight, roots dry weight and proline accumulation. Moreover, the growth reduced as a result of water shortage due to water stress except roots length which were increased. Proline concentrations increased by rising the period of water withholding from 3 to 5 days compared to continuous irrigated treatment (control). Meanwhile, salicylic acid stimulates the accumulation amount of proline in stressed plant leaves. The Iraqi cultivar exceed the American cultivar in: seed germination percentage, plant length, number of flowers, root fresh weight, root length and roots dry weight, while the American cultivar surpasses the Iraqi cultivar in: number of branches, number of leaves, leaves area, shoot fresh weight, shoot dry weight and proline concentration.

الكلمات الرئيسية

المراجع

Ahenkora K, Adu-Dapaah HK, Agyemang A (1998) Selected nutritional components and sensory attributes of cowpea (Vigna unguiculata L.] Walp.) leaves. Plant Foods Hum Nutr 52:221-229.

Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and soil, 39 (1), 205-207.

Ceccarelli S, Grando S. (1996). Drought as a challenge for the plant breeder. J. Plant Growth Reg. 20: 149-155.

Duncan, D.R. (1995). Multiple range and multiple F. test. J. Biometrics 11:1-42. SAS (2016). Statistical analysis system (SAS), Users Guide,ver. 9.4, SAS Institute, USA.

Hall AE, Cisse N, Thiaw S, Elawad HOA, Ehlers JD, Ismail A, Fery R, Roberts P, Kitch LW, Murdock LL, Boukar O, Phillips RD, McWatters KH (2003) Development of cowpea cultivars and germplasm by the Bean/Cowpea CRSP. Field Crops Res 82:103–134

Karamanos AS. (1980). Water stress and leaf growth of field beans (Vicia faba) in the field: Leaf number and total area. Ann. Bot. 42: 1393-1402.

Naidu BP, Paleg LG, Jones GP. 1992. Nitrogenous compatible solutes in drought stressed Medicago spp. J Phytochem. 31: 1195-1197.

Nielson SS, Brandt WE, Singh BB (1993) Genetic variability for nutritional composition and cooking time of improved cowpea lines. Crop Sci 33:469-472.

Nonami H. 1998. Plant water relations and control of cell elongation at low water potentials. J. Plant Res. 111: 373-382.

Shakirova FM (2007). Role of hormonal system in the manifestation of growth promoting and anti-stress action of salicylic acid. In: Hayat S, Ahmad A (eds) Salicylic acid, A plant hormone. Springer, Dordrecht, Netherlands pp. 69-89.

Tarawali SA, Singh BB, Gupta SC, Tabo R, Harris F, Nokoe S, Fernلndez-Rivera S, Bationo A, Manyong VM, Makinde K, Odion EC (2002). Cowpea as a key factor for a new approach to integrated crop-livestock systems research in the dry savannas of West Africa. In: Fatokun CA, Tarawali SA, Singh BB, Kormawa PM, Tamo M (eds) Challenges and Opportunities for Enhancing Sustainable Cowpea Production. International Institute of Tropical Agriculture, Ibadan, Nigeria, pp 233–251.

Thomas H. (1997). Drought resistance in plants. In: Basra AS, Basra RK. (eds). Mechanisms of environmental stress resistance in plants. Harwood Academic Publishers pp 1-42.