Sero-diagnosis of Bovine tuberculosis using a rapid lateral flow test and ELISA in dairy cattle in Egypt

نوع المستند : المقالة الأصلية

المؤلفون

1 Department of bacterial diagnostic product (TB), Veterinary Serum and Vaccine Research Institute, Cairo, Egypt.

2 .Professor of Microbiology Department, Faculty of Veterinary Medicine Beni-Suif University.

3 Microbiology Department, Faculty of Veterinary Medicine Beni-Suif University.

4 Department of Microbiology, Faculty of Veterinary Medicine, Matrouh University

5 Head of Genetic Engineering Research Department (GERD) Veterinary Serum and Vaccine Research Institute, Cairo, Egypt..

المستخلص

            Mycobacterium bovis (M. bovis), the cause of bovine tuberculosis (BTB) in cattle, remains a major zoonotic and economic problem in many countries. This study aimed to apply different serological tests for detection of bovine tuberculosis as a complementary for intradermal tuberculin test. The tested 4250 cattle in different Egyptian governorates by single intradermal cervical comparative tuberculin test (SICCT), 90 (2.1%) cattle reacted positively. The postmortem (PM) examination of the positive reactors revealed 65 (72.2%) out of 90 slaughtered cattle with visible lesions (VL) while the other 25 (27.8%) reactors showed no visible lesions (NVL). The visible lesions detected in head were 10 (15.4%) out of the 65 VL, in respiratory system were 32 (49.2%), in the digestive system were 5 (7.7%), in mixed infection were 11 (16.9%), and in generalized infection were 7(10.8%). The bacteriological examination of processed samples from the 90 slaughtered cattle revealed 55 M. bovis (61.1%); 51 (78.5%) were from 65 VL and 4 (16.0%) were from 25 NVL. Enzyme linked immune sorbent assay (ELISA) was used for the tuberculin positive animals’ sera and gave 36 (55.4%) out of 65 cattle with and 2 (8%) out of 25 cattle with NVL by a total 38 (42.2%) out of the 90 tuberculin positive animals. The results of lateral flow test gave 32 (49.2%) out of 65 cattle with VL and 1 (4%) out of 25 cattle with NVL by a total 33 (36.7%) out of the 90 tuberculin positive animals.

المراجع

Abdullah Kaya, Hasan İcen, Nevin Tuzcu, Aynur Şimsek, Simten Yesilmen, Akın         Kochan, İbrahim Yildirim, Adil Alan, (2015). Comparison of Tuberculin Skin Test,           IFN-γ Assay, Real Time PCR and Lateral Flow Rapid Test in Diagnosis of Field            Outbreaks of Bovine Tuberculosis. Kafkas Univ. Vet Fak Der21 (5): 739-743, 2015.
Abuo – Eisha, A.M., El – Attar, A.A., and El – Sheary, M.N., (1995). Bovine and atypical             mycobacterial infection of cattle and buffalos in Port Said Province, Egypt. Assiut Vet.    Med. J., 47: 152 -162.
Adawy, A.T., (1986). Studies on tuberculosis in slaughtered cattle. Ph. D. Thesis, Bacteriology, Immunology, Mycology, Fac. Vet. Med., Cairo University. 
Akçay, E., (2000). Sığır tüberkülozunun teşhisinde kullanılan gamma interferon testi ile             ELISA’nın karşılaştırmalı değerlendirilmesi. Doktora Tezi. Ankara Üniversitesi Sağlık            Bilimleri Enstitüsü, Ankara, 2000.
Amadori, M., Tameni, S., Scaccaglia, P.I., Archetti, I.I. and Quondam, R.G., (1998).    Antibody test for identification of Mycobacterium bovis infected bovine herds. J. Clin.          Microbiol., 36:566- 568.
Ameni, G. and Erkihun, A., (2007). Bovine tuberculosis on small – scale dairy farms in             Adam Town, Central Ethiopia, and farmer awareness of the disease. Rev. Sci. Tech. Off. Int. Epiz., 26(3): 711- 719.
Asiak, I.E., Ohore, O.G., Emipke, B.O., Abantan, O.O. and Ockiya, M.A., (2007). The use of ELISA in the detection of bovine tuberculosis in slaughtered trade cattle and sedentary herds in South West Nigeria. J. Anim. Vet. Adv. 6: 883-886, 2007.
Awah-Ndukum, J. Kudi, A.C. Bradley, G. Ane-Anyangwe, I.N., Fon-Tebug, S.    Tchoumboue, J., (2010). Prevalence of bovine tuberculosis in abattoirs of the Littoral           and Western highland regions of Cameroon: a cause for public health concern. Veterinary   Medicine International 2010, 8 pages; doi:10.4061/2010/495015.
Borna, M., Penelope, V., Bongo, N., Colette, D., Irene, S., Beatric, M., Bruno, O.,           Esther, S. and Jakob, Z., (2009). Bayesian Receiver Operating Characteristic          Estimation of multiple tests for Diagnosis of Bovine tuberculosis in Chadian Cattle. Plose      One 4(12): 1371- 1381.
Brahma, Derhasar, Deepti Narang, Mudit Chandra, Gursimran Filia, Amarjit Singh,             Sikh Tejinder Singh, (2019). Diagnosis of Bovine Tuberculosis by Comparative            Intradermal Tuberculin Test, Interferon Gamma Assay and esxB (CFP-10) PCR in Blood       and Lymph Node Aspirates. Open Journal of Veterinary Medicine, 2019, 9, 55-65
Casillas, C.R., Elizondo, G.V. and Diaz, C.A., (1995). Comparicion del ELISA con la      tubercilinizacion en el diagnostico de la tuberculosis bovina. Tec. Pecu. Mex. 33: 148-           158, 1995.
Chambers, M. A., (2013). Review of the Diagnosis of Tuberculosis in Non-Bovid                        Wildlife Species Using Immunological Methods. Transboundary and Emerging                     Diseases Volume 60, Issue Supplement s1, 14–27.
Cleaveland, S., Shaw D.J. Mfinanga, S.G., Shirima, G.,Kazwala, R.R., Eblate,E., and    Sharp, M., (2007). Mycobacterium bovis in rural Tanzania: risk factors for infection in          human and cattle populations. Tuberculosis, 87(1):30-43.
Collee, J.G., Fraser, A.G., Marmion, B.P. and Simmons, A., (1996). Mackie &    MacCartney Practical Medical Microbiology 14th Ed., 838–841.Churchill Living Stone,      New York, Edinburgh, London.
Collins, J.D., (2006). Tuberculosis in cattle: strategic planning for the future. Veterinary             Microbiology, vol. 112, no. 2-4, pp. 369-381.
Cortina, N., and Vera, A., (1986). Prevalence of a typical mycobacteria. Rev. cubana de            ciencias veterinarias, 17(314):163-168.
Danbirni, S., Sackey, A.K.B., Kudi, A.C., Okaiyeto, S.O., and Pewan, S.B., (2009). A      comparison of one-step antigen rapid bovine tuberculosis antibodies test sensitivity to      postmortem gross lesions in diagnosing bovine tuberculosis in a dairy herd in Kaduna       state. Research Journal of Dairy Sciences 3 (2-4): 3234.
Danbirni, S., Okaiyeto, S.O., Bature C. and Moris, A., (2013). Field Determination of    Tuberculosis Prevalence in a Herd of Cattle Using Tuberculin and Quicking® Bovine      Tuberculosis Antibody Rapid Tests in Jalingo, Nigeria. J. Vet Adv, 3(1): 20-23.
De La Rua-Domenech, R., Goodchild, A.T., Vordermeier, H.M., Hewinson, R.G.,          Christiansen, K.H. and Cliftonhadley, R.S., (2006). Ante mortem diagnosis of       tuberculosis in cattle: a review of the tuberculin tests, gamma-interferon assay and other       ancillary diagnostic techniques. Research in Vet Science, vol. 81, no. 2, pp. 190-210.
Durnez, L.; Katakweba, A.; Sadiki, H.; Katholi, C. R.; Kazwala, R. R.; Machang'u, R.            R.; Portaels, F.; Leirs, H., (2011). Mycobacteria in terrestrial small mammals on cattle         farms in Tanzania. Vet Medicine International; 2011. 2011:Article ID 495074. 74 ref.
El Battawy, E.H., (2008). Bacteriological characterization, molecular and immunological          diagnostic assays for Mycobacterium infection in some animals. Ph.D. Thesis Minufiya          University, Sadat City branch Faculty of Veterinary Medicine, Dep. of Bacteriology           Immunology and Mycology.
El- Sabban , M.S.,  Lotfy ,O., Awad , W.M., Soufi, H.S., Mikhail,  D.G., Hammam,         H.M., Dimitri , R.A. and Gergis, S.M., (1992). Bovine tuberculosis and it is extent of         spread as source of infection to man and animals in Egypt. Proc. of the Int. Conf. on Ani.     TB in Middle East, Cairo, 198- 21.
Fawzy Riyad El-Seedy, Ismail Abd-El Hafeez Radwan, Walid Hamdy Hassan, Essam             Amin Nasr, Ahmed Hussein Abed and Ihap Mohamed Ibrahim Moussa, (2013). The   correlation between M. bovis isolation and ELISA using PPD-B and ESAT6- CFP10   mixture on the sera of tuberculin reactor cattle and buffaloes. Journal of Food,     Agriculture & Environment Vol.11 (1): 489-494. 2013.        
Gobena, A., Glyn, H., Abraham, A., Douglas, Y., and Martin, V., (2008). Appraisal of   interpretation criteria for the comparative intradermal tuberculin test for the diagnosis of            bovine tuberculosis in central Ethiopia. Clin. Vaccine Immunol. 8:1272  1276.
Good, M., and Duignan, A., (2011). “Perspectives on the history of Bovine TB and the   role of tuberculin in Bovine TB eradication,” Vet. Med. Int., vol. 11 pages, 2011. Article          ID 410470.
Grist, A., (2008). Bovine meat inspection-Anatomy, Physiology and Disease Conditions.           2nd ed. Notting-ham University Press, Nottingham, UK. 296 pp.
Guindi, S.M., Lotfy, A. O., Hantera, M., and Salah El – Din, M., (1965). The        tuberculosis test specificity.  Proc. 6th  Annu. Vet. Congress.
Huitema, H., (1994). Tuberculosis in animals and man. 1st  Ed. Publication of the Royal Netherlands Tuberculosis Association (KNCV). 79.
Jang, Yun-Ho, Kim, Tae-woon, Jeong, Min Kyu, Yoon Jeong Seo, Soyoon Ryoo, Chan            Ho Park, Sin seok Kang, Young Ju Lee, Soon-Seek Yoon, and Jae Myung Kim       (2020). Introduction and application of the interferon-ɣ Assay in the National Bovine Tuberculosis Control Program in South Korea. Frontiers in Vet research, April 2020-    Volume 7- Article 222.
Kalaf, J.M., Salbouk, A.J., and Salman, S.S., (2014). Detection of bvine tuberculosis in             Wasit City by the use of comparative intradermal tuberculin test and antigen rapid bovine     TB Ab test. AL- Qadisiya J. Vet. Med. Sci. 13(2):58-62.
Keskin, O., and İzgür, M., (1996). Sığır tüberkülozisi’nin teşhisinde ELISA’nın   kullanılması ve allerjik yöntemle karşılaştırılmasi. I. Uluslararası Veteriner Mikrobiyoloji Kongresi. 92-93, İstanbul, 1996.
Keskin, O., (2001). Sığır tüberkülozisinin teşhisinde ELISA’nın kullanılması ve allerjik yöntemle karşılaştırılması. Vet. Hek. Mikrobiyol. Derg. 1: 11-18, 2001.
Kilian, H., (1982). Occurrence of mycobacteria in lymph nodes and muscles of slaughtered       cattle and swine with reference to isolated tuberculosis of lymph nodes. Inaugural     Dissertation, Fach berich Veterinar medizin, Freie universital Berlin, 112.
Krambovitis, E., (1986). Detect- ion of antibodies to mycobacterium tuberculosis plasma          membrane antigen by Enzyme Linked Immunosorbent Assay. J. Med. Microbiol., 21: 257-264.
Lotfy, O., Khater, A.R., Guindis, S.M., (1960). An investigation on tuberculosis Umakenan Village, Egypt, U.A.R. J. Egypt, Vet. Med. Ass., 20: 105- 112.
Lyashchenko, K.P., Pollock, J.M., Colangeli, R., Gennaro, M.L., (1998). Diversity of    antigen recognition by serum antibodies in experimental bovine tuberculosis. Infect          Immun 66:5344– 5349.
Mcnair, J., Corbeet, D.M., Cirvin, R.M., Mackie, D.P., and Pollock, J.M., (2001).          Characterization of the early antibody response in bovine tuberculosis :MPB83 in early        target with diagnosis potential. Scand. J. Immuniol., 53: 365 – 371.
Mohamed, M., Moussa, I.M., Mohamed, K.F., Samir, A., Nasr, E.A., Ashgan, M.H.,      Salim Al-Rejaie; Hatem, M.E., (2011).BACTEC MGIT 960 TM system             for screening of Mycobacterium tuberculosis complex among cattle. African Journal of       Biotechnology; 2011. 10(63):13919-13923. 14 ref.
Monaghan, M.L., Doherty, M.L., Collins, J.D., Kazda, J.F., and Quinn, P.J., (1994).      The tuberculin test. Vet. Microbiol. 40: 111-124, 1994.
Mosaad, A. A.; Abdel-Hamed, A. S.; Fathalla, S. I.; Ghazy, A. A.; Elballal, S.;    Elbagory, A.; Mahboub, H.; Gaafar, K.; Elgayar, K. E.; Mohamed, A. S.;  Amin,  A.      I.; Akeila, M. A. and Abdelrahman, H. A. (2012).   Sensitive and Specific Diagnostic       Assay for Detection of Tuberculosis in Cattle. Global Veterinaria, 8(6): 555-564.
Naglaa, R.A., Hassan, (2008). Emergency Mycobacterium tuberculosis complex organisms: advances in diagnosis and drug resistance.  Ph. D. Thesis, Bacteriology,    Immunology, Mycology, Fac. Vet. Med., Cairo University.
Nasr, E.A., (1997). Studies on atypical mycobacterial microorganisms. Ph.D. Thesis,       (Bacteriology, Immunology, Mycology), Fac.Vet. Med., Zagazig           University.
Nasr, E.A., Marwah, M., Lilian, F.S., MelikaAbeer, A., Tammam, Seham, F., Gorge,    (2016). Comparison of tuberculin skin test and lateral flow rapid test for detection of        bovine tuberculosis in dairy cattle. 8th Scientific Congr. of Egypt. Soc. For Anim. Manag.   23-27 August, 2016: 1- 16.
Essam Amin Nasr, Walid Hamdy Hassan, and Hassan Mohamed  Moussa, (2018). A   qualitative immunoassay as complementary test with tuberculin skin test for detection of       tuberculosis in dairy cattle. J. of Vet. Med. Res. 2018, 25 (2): 182-190.
Nassau, E., Parsons, E. R., and Johnson, G. D., (1976). The detection of antibodies to    Mycobacterium tuberculosis by microplate enzyme linked immunosorbent assay      (ELISA). Tubercle 57:67-70.
Neill, S.D., Bryson, D.B., and Pollock, J.M., (2001). Pathogenesis of tuberculosis in cattle.        J. Tuberc. 81: 79-86, 2001.
OIE, (2018). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.
            Chapter 3.4.6.Bovine tuberculosis 1058-1074,2018.
O’ Reilly, L. M., (1992). Specificity and sensitivity of tuberculin test. Proc. Int. Conf.     Anim. Tuberculosis, General Organization of Vet. ser., Cairo, Egypt, 87 – 139.
Pereira, H. de M.; Santos, H.P.; Bezerra, D.C., Pereira, D.P., (2009). Occurrence of      tuberculosis in cattle raised in the municipality of Rita, Maranhao, Brazil. Ciencia Animal             Brasileira; 2009. 10(Supplement 1):508-512. 16 ref.
Placket, P., Ripper, J., Corner, L.A., Small, K., Witte, K., Melville, L., Hides, S. and     Wood, P.R., (1989). An ELISA for the detection of anergic tuberculous cattle. Aust. Vet.            J. 66: 15-19, 1989.
Pollock, J.M., Welsh, M.D., and McNair, J., (2005). Immune responses in bovine           tuberculosis: Towards new strategies for the diagnosis and control of disease. Vet.            Immun. Immunopathol. 108: 37-43, 2005.
Retmal, P.I., Abalos, P.E., (2004). Comparison of the bovine gamma interferon assay with         traditional techniques for detecting infection with Mycobacterium bovis. Revista-       Colombiana-de-Ciencias-pecuarias.2004; 17(3):223-230.
Ritacco, V., Lopez, B., Barrera, L., Nader, A., Fliess, E. and Kantor, I.N., (1990).          Further evaluation of an indirect enzyme linked immunosorbent assay for the diagnosis of           bovine tuberculosis. J. Vet. Med. 37: 19-27, 1990.
 Sayin, Z., and Erganis, O., (2013). Diagnosis of Bovine Tuberculosis by PPD-ELISA and             Sonication-ELISA. Israel Journal of Veterinary Medicine,Vol. 68 (3) September 2013.
Schiller, I., Oesch, B., Vordermeier, H.M., Palmer, M.V., Harris, B.N., Orloski, K.A., Buddle, B.M., Thacker, T.C., Lyashchenko, K.P. and Waters, W.R., (2010). Bovine        tuberculosis: a review of current and emerging diagnostic techniques in view of their   relevance for disease control and eradication. Transboundary and Emerging Diseases,   vol. 57, no.4, pp. 205-220. PMid: 20561288.
Shannon, C Duffy, Sreenidhi Srinivasan, Megan A Schilling, Tod Stuber, Sarah N        Danchuk, Joy S Michael, Manigandan Venkatesan, Nitish Bansal, Sushila Maan,   Naresh Jindal, Deepika Chaudhary, Premanshu Dandapat, Robab Katani,    Shubhada Chothe, Maroudam Veerasami, Suelee Robbe-Austerman, Nicholas          Juleff, Vivek Kapur, Marcel A Behr, (2020). Reconsidering Mycobacterium bovis as a       proxy for zoonotic tuberculosis: a molecular epidemiological surveillance study. Lancet             Microbe 2020; 1: e66–73.
Shenzhanov, K.T., Bulashev, A.K., and Bakırova, G.A., (2003). Using monoclonal         antibodies in developing ELISA for diagnosing bovine tuberculosis. Vet. Archiv. 73: 81- 93, 2003.
Shirma, G.M., Kazwala, R.R., and Kambarage, D.M., (2003). Prevalence of bovine       tuberculosis in cattle in different farming systems in Eastern zone of Tanzania. Prev. Vet. Med.57:167-172.
Shitaye, J. E.; Getahun, B.; Alemayehu, T.; Skoric, M.; Treml, F.; Fictum, P.; Vrbas, V. and Pavlik, I., (2006). A prevalence study of bovine tuberculosis by using abattoir            meat inspection and tuberculin skin testing data, histopathological and IS6110 PCR     examination of tissues with tuberculous lesions in cattle in Ethiopia. Veterinarni       Medicina, 51:512-522.
Smyth, A.J., Welsh M.D., Girvin, R.M., and Pollock, J.M., (2001). In vitro           responsiveness of γδ T cells from Mycobacterium bovis infected cattle to mycobacterial      antigens predominant involvement of WC1+ Cells. Infection and Immunity, 69: 89 – 96.
Sven, D.C., Parsons1, Kevina McGill, Mairead, B., Doyle, Wynand, J., Goosen, 1.,        Paul, D., van Helden, Eamonn Gormley, (2016). Antigen-SpecificIP-10 Release Isa        Sensitive Biomarker of Mycobacterium bovis Infection in Cattle. Journal pone 0155440      May11, 2016.
Tammemagi, L., Simmons, G.C., Kelman, R., and Hall, W.T.K., (1973). A study of        tuberculosis like lesions in cattle slaughtered in Queensland meat works.  Aust. Vet. J.,           49: 507- 511.
Tewodros Fentahun, and Girja Luke, (2012). Diagnostic Techniques of Bovine Tuberculosis: A Review. African Journal of Basic & Applied Sciences 4 (6): 192-199,          2012.
Thorns, C.J., and Morris, J.A., (1983). The immune spectrum of Mycobacterium bovis infections in some mammalian species. Vet. Bull., 53 (6):543–547.
Vordermeier, M., Goodchild, A., Clifton-Hadley, R., and De la Rua, R., (2004). The      interferon-gamma field trial: background, principles and progress. Vet. Rec. 155: 37-38,         2004.
Wernery,  U., J. Kinne, K. L. Jahans et al., (2007). Tuberculosis outbreak in a dromedary         racing herd and rapid serological detection of infected camels,” Vet. Microbio., vol. 122,            no. 1-2, pp. 108–115.
Whelan C, Shuralev E, Kwok HF, Kenny K, Duignan A, Good M, Davis WC, Clarke J            (2011). Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation           of Mycobacterium bovis-infected animals deemed negative or inconclusive by the single    intradermal comparative tuberculin skin test. J Vet Diagn Invest 23:499–503.
Zivkovic, J.S., Berko, M., and Hadziosmu, V., (1984). Suitability for human consumption        of meat from tuberculin positive cattle. Vet. Bull. (1985), 55 (10):6718.
Zschoc, M., Palatky, R., And Eskens, U., (1990). Tubercular lymph nodes. Culturing      results for slaughter cattle for the years 1989. Flesch  Wirtschaft,  70: 285 – 286.  
 

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